5-aza-dC体外诱导兔骨髓间充质干细胞分化为胰岛细胞的研究

doi:10.11843/j.issn.0366-6964.2014.09.022

Abstract

Abstract:

To explore the relationship between the changes of DNA methylation level and the differentiation of BMSCs，the effect on cell viability of DNA methylation inhibitors (5-aza-dC) in different concentrations at different times was detected by MTT method.The cells were treated with 5-aza-dC at the concentration of 1.0 μmol•L^-1 and differentiate into islet cells combining with DMSO and nicotinamide，which was compared with the induction group that was not treated with 5-aza-dC with Dithizone staining method and the immunofluorescent staining method in the key regulatory genes of pancreatic islet (PDX-1).The results showed that the proper concentration of 5-aza-dC for the treatment of rabbit BMSCs ranges from 0.2 to 1.0 μmol•L^-1.The combine use of 5-aza-dC at the concentration of 1.0 μmol•L^-1 and DMSO，nicotinamide could promote the expression of the key regulatory genes of pancreatic islet (PDX-1) and established an efficient system for the differentiation of BMSCs into islet cells.It reveals that the degree of methylation could influence the differentiation of BMSCs into islet cells and the low degree of methylation could promote the expression of specific gene of pancreatic islet (PDX-1).

CLC Number:

LYU Jing-yu，XU Xiao-ting，CHEN Xiao-pei，ZHAI Ming-sheng，FENG Jian-kun，YU Wen-hao，WANG Xin-zhuang. Differentiation of Rabbit Bone Mesenchymal Stem Cells into Islet Cells by 5-Azacytidine in vitro[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(9): 1538-1543.

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Differentiation of Rabbit Bone Mesenchymal Stem Cells into Islet Cells by 5-Azacytidine in vitro

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